New names inPhanerogamae 6

94 new nomenclatural combinations are proposed, resulting mostly from the study of taxonomic problems on the generic level. The reasons for the acceptance of the generic rank are given in the case ofAcetosa, Pulegium and of some sections ofOrobanche s. l. andSilene s. l. The taxonomic rank ofGentiana blepharophora Bordzi?. from the Caucasus has been revised and the taxon is classified as a subspecies ofGentianopsis ciliata. Nomenclatural problems of not numbered “species” inHoffmann's “Flora Deutschlands” from 1800 are demonstrated on the example ofArmeria elongata.     

A newHottarum (Araceae) species from Borneo

The new speciesH. lucens Bogner differs fromH. truncatum (M. Hotta)Bogner etNicolson: The leaf blades are narrower, the spadix is adnate to the spathe for two third of its length, i.e. with all of its female and half of its male part. The latter has (fertile) stamens only on its front and staminodes on its back side. Further staminodes occur between the male and female flowers.     

Stoicheiometry of dicyclohexylcarbodiimide-ATPase interaction in mitochondria

1. The oligomeric dicyclohexylcarbodiimide (DCCD)-binding protein of mitochondrial ATPase was studied using (a) the relationship between [¹⁴C]DCCD binding and inhibition of ATPase activities and (b) the analysis of the kinetics of inhibition. 2. The [¹⁴C]DCCD binding to bovine heart mitochondria is linearly proportional to the inhibition of ATP hydrolysis up to a 50% decrease of the original activity resulting in 0.6 mol DCCD bound covalently to the specific inhibitory site (Hous?t?k, J., Svoboda, P., Kopecký, J., Kuz?ela, S?. and Drahota, Z. (1981) Biochim. Biophys. Acta 634, 331–339) per mol of the fully inhibited enzyme. 3. Kinetics of the inhibition of both the ATPase activity (heart and liver mitochondria) and ADP-stimulated respiration (liver) reveal that 1 mol DCCD per mol ATPase eliminates both the synthetic and the hydrolytic activities. It is inferred that the activity-binding correlation underestimates the number of DCCD-reactive sites. 4. The second-order rate constant of the DCCD-ATPase interaction (k) is inversely related to the concentration of membranes, indicating that DCCD reaches the inhibitory site by concentrating in the hydrophobic (phospholipid) environment. 5. At a given concentration of liver mitochondria, comparable k values are obtained both for the inhibition of ATP hydrolysis (k=5.35·10²M^?1·min^?1) and ADP-stimulated respiration (k=5.67·10²M^?1·min^?1). 6. It is concluded that both the synthetic and the hydrolytic functions of ATPase are inhibited via a common single DCCD-reactive site. This site is represented by one of the several polypeptide chains forming the oligomer of the DCCD-binding protein. The inhibitor-ATPase interaction does not exhibit cooperativity, indicating that the preferential reactivity towards DCCD is an inherent property of the inhibitory site.     

Reclassifications and new names for some European Phanerogams

On the basis of taxonomic and nomenclatural revisions of various plant groups 37 new nomenclatural combinations are proposed mostly for European phanerogamous taxa (21 for species, 14 for subspecies, 2 for hybrids.)Medicula Medicus,Pseudofumaria Medicus andThlasidia Rafin. are accepted as taxonomically justified genera and correct generic names.Dactylorhiza longebracteata (F. W. Schmidt)Holub is proposed instead ofD. fuchsii (Druce) Soó, andHylotelephium triphyllum (Haworth) Holub isstead ofH. purpureum (L.)Holub.     

Some neglected subspecies names from Schübler and Martens,Flora von Würtemberg (1834)

A list including 114 selected subspecies names from the bookSchübler andMartens, Flora von Würtemberg, 1834 is given. Twenty of them are very important for the present taxonomic classification.     

Validation of generic names inLycopodiaceae: with a description of a new genusPseudolycopodiella

Four earlier proposed and invalidly published generic names ofLycopodiaceae are validated:Lateristachys (type:Lycopodium laterale R. Br.),Lycopodiastrum (type:Lycopodium casuarinoides Spring),Pseudodiphasium (type:Lycopodium volubile G. Forster) andPseudolycopodium (type:Pseudolycopodium densum (Rothm.) Holub). A new genusPseudolycopodiella (type:Lycopodium carolinianum L.) is described. New nomenclatural combinations are proposed for 17 species of these genera and ofLycopodiella.     

A rapid method for the determination of the base composition of bacterial DNA

A rapid method to evaluate the (G+C)-content of bacterial DNA is described. About 2 × 10⁸ cells are lysed by the combined action of detergents and enzymes and put into a CsCl-gradient without any purification. Up to five samples may be run at once. The high background absorption of cell-derived material other than DNA is overcome by novel collimating optics.     

Purification and partial characterization of the 17 kDa sperm coating protein from boar seminal plasma.

Sperm coating proteins of 16, 17, and 19 kDa have been purified from boar seminal plasma. The 17 kDa protein has been identified as an antigen recognized by monoclonal antibody ACR.3 and is thus identical to low molecular mass zona pellucida binding protein from boar spermatozoa (Moos et al., 1990). The 17 and 19 kDa proteins are glycosylated and tend to form hetero-complexes. The 17 kDa ACR.3 antigen is sequentially released from the sperm cell surface during capacitation and, after induction of the acrosome reaction, the 16 kDa form was also observed. Immunocytochemical studies on boar reproductive tissues have suggested that the seminal vesicle epithelium may be the source of these proteins.     

Enhanced Rate of Expression and Biosynthesis of Neuropeptide Y After Kainic Acid-Induced Seizures

Recent studies have shown marked increases in brain content of neuropeptide Y (NPY) after seizures induced by intraperitoneal injection of kainic acid and after pentylenetetrazole kindling in the rat. We have now investigated possible changes in the rate of biosynthesis of NPY after kainic acid treatment, by using pulse-labeling of the peptide and by determining prepro-NPY mRNA concentrations. For pulse labeling experiments, [3H]tyrosine was injected into the frontal cortex, and the incorporation of the amino acid into NPY was determined after purifying the peptide by gel filtration chromatography, antibody affinity chromatography, and reversed-phase HPLC. At 2 and 30 days after kainic acid treatment, the rate of tyrosine incorporation was enhanced by approximately 380% in the cortex. In addition, concentrations of pre-pro-NPY mRNA were determined in four different brain areas by hybridization of Northern blots with a complementary 32P-labeled RNA probe 2, 10, 30, and 60 days after kainic acid treatment. Marked increases were observed in the frontal cortex (by up to 350% of controls), in the dorsal hippocampus (by 750%), and in the amygdala/pyriform cortex (by 280%) at all intervals investigated. In the striatum only a small, transient increase was observed. The data demonstrate increased expression of prepro-NPY mRNA and an enhanced rate of in vivo synthesis of NPY as a result of seizures induced by the neurotoxin kainic acid.     

Eight newRubus species described from Czech Republic

Eight newRubus species are described from the area of Czech Republic (western Czechoslovakia), four of them belonging to ser.Discolores, and one by one to subsect.Rubus, ser.Rhamnifolii, ser.Micantes and ser.Hystrices. *** DIRECT SUPPORT *** AAY02002 00005